ARG41393
anti-TYROBP / DAP12 antibody
anti-TYROBP / DAP12 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes TYROBP / DAP12 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TYROBP / DAP12 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human TYROBP / DAP12. (C-KQRITETESPYQE) (NP_003323.1; NP_937758.1) |
| Conjugation | Un-conjugated |
| Alternate Names | TYRO protein tyrosine kinase-binding protein; DNAX-activation protein 12; DAP12; PLOSL; KARAP; KAR-associated protein; Killer-activating receptor-associated protein |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43914 Human TYRO protein tyrosine kinase-binding protein |
|---|---|
| Gene Symbol | TYROBP |
| Gene Full Name | TYRO protein tyrosine kinase binding protein |
| Background | This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Mar 2010] |
| Function | Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 12 kDa |
| PTM | Tyrosine phosphorylated. [UniProt] |
