ARG63110
anti-Tenascin C antibody [T2H5]
anti-Tenascin C antibody [T2H5] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [T2H5] recognizes Tenascin C |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone T2H5 recognizes Tenascin C, a large hexameric extracellular matrix glycoprotein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | T2H5 |
| Isotype | IgG1 |
| Target Name | Tenascin C |
| Antigen Species | Human |
| Immunogen | Protein preparation from a homogenate of a human mammary tumour specimen. |
| Conjugation | Un-conjugated |
| Alternate Names | Cytotactin; HXB; DFNA56; Myotendinous antigen; Hexabrachion; GP; Tenascin-C; Neuronectin; GMEM; TN; 150-225; JI; TN-C; GP 150-225; Glioma-associated-extracellular matrix antigen; Tenascin |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Perform heat mediated antigen retrieval before IHC-P staining step. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TNC |
| Gene Full Name | tenascin C |
| Background | This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011] |
| Function | Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 241 kDa |
Clone References
Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1.
WB, IHC-P / Human
Tenascin-C is an innate broad-spectrum, HIV-1-neutralizing protein in breast milk.
WB /
Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways?
ICC/IF / Human
Tenascin-C as a prognostic biomarker in osteosarcoma?
IHC-P / Human
