ARG57177

anti-Thioredoxin Reductase 1 antibody [30F1]

anti-Thioredoxin Reductase 1 antibody [30F1] for ICC/IF,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [30F1] recognizes Thioredoxin Reductase 1
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 30F1
Isotype IgG2b, kappa
Target Name Thioredoxin Reductase 1
Antigen Species Human
Immunogen Recombinant fragment around aa. 161-647 of Human Thioredoxin Reductase 1
Conjugation Un-conjugated
Alternate Names Thioredoxin reductase 1, cytoplasmic; EC 1.8.1.9; Gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; TR; TXNR; TRXR1; KM-102-derived reductase-like factor; TR1; GRIM-12; Thioredoxin reductase TR1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7296 Human TXNRD1

Swiss-port # Q16881 Human Thioredoxin reductase 1, cytoplasmic

Gene Symbol TXNRD1
Gene Full Name thioredoxin reductase 1
Background This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]
Function Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. [UniProt]
Calculated MW 71 kDa
PTM The N-terminus of isoform 5 is blocked.
ISGylated.