ARG55981
anti-Thyroglobulin antibody [2H11]
anti-Thyroglobulin antibody [2H11] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [2H11 ] recognizes Thyroglobulin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2H11 |
| Isotype | IgG1, kappa |
| Target Name | Thyroglobulin |
| Antigen Species | Human |
| Immunogen | Human thyroid follicular cells. |
| Conjugation | Un-conjugated |
| Alternate Names | AITD3; Tg; TGN; Thyroglobulin |
Application Instructions
| Application Suggestion |
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| Application Note | Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TG |
| Gene Full Name | thyroglobulin |
| Background | Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009] |
| Function | Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). [UniProt] |
| Cellular Localization | Cytoplasmic and secreted |
| Calculated MW | 305 kDa |
| PTM | Sulfated tyrosines are desulfated during iodination. |
