ARG66205

anti-Topoisomerase II antibody [SQab1727]

anti-Topoisomerase II antibody [SQab1727] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

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Overview

Product Description

Recombinant Rabbit Monoclonal antibody [SQab1727] recognizes Topoisomerase II

Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1727
Target Name Topoisomerase II
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Topoisomerase II.
Conjugation Un-conjugated
Alternate Names DNA topoisomerase II, alpha isozyme; DNA topoisomerase 2-alpha; TOP2; EC 5.99.1.3; TP2A

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 ‐ 1:200
WB1:2000 - 1:5000
Application Note IHC: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21973 Mouse TOP2A

GeneID: 7153 Human TOP2A

Swiss-port # P11388 Human DNA topoisomerase 2-alpha

Swiss-port # Q01320 Mouse DNA topoisomerase 2-alpha

Gene Symbol TOP2A
Gene Full Name topoisomerase (DNA) II alpha
Background Topoisomerase II, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
Function Topoisomerase II controls of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. [UniProt]
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Calculated MW 174 kDa
PTM Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

Customer's Feedback

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Review for anti-Topoisomerase II antibody [SQab1727]

Application:WB

Sample:293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC