ARG65462
anti-Transferrin antibody [HTF-14] (low endotoxin)
anti-Transferrin antibody [HTF-14] (low endotoxin) for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,ELISA,Radioimmunoassay,Functional study and Human,Pig,Rabbit
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [HTF-14] recognizes Transferrin |
|---|---|
| Tested Reactivity | Hu, Pig, Rb |
| Species Does Not React With | Cow, Dog, Hrs, Sheep |
| Tested Application | ELISA, FuncSt, ICC/IF, IHC-P, RIA, WB |
| Specificity | The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HTF-14 |
| Isotype | IgG1 |
| Target Name | Transferrin |
| Antigen Species | Pig |
| Immunogen | Purified porcine transferrin. |
| Conjugation | Un-conjugated |
| Alternate Names | Beta-1 metal-binding globulin; Siderophilin; Transferrin; PRO1557; TFQTL1; Serotransferrin; PRO2086 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by precipitation and chromatography. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TF |
| Gene Full Name | transferrin |
| Background | This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009] |
| Function | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 77 kDa |
Clone References
Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface.
Transferrin coated nanoparticles: study of the bionano interface in human plasma.
Dot /
Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity.
Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.
