ARG63731

anti-Triosephosphate isomerase antibody

anti-Triosephosphate isomerase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Triosephosphate isomerase
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Triosephosphate isomerase
Antigen Species Human
Immunogen C-LKPEFVDIINAKQ
Conjugation Un-conjugated
Alternate Names EC 5.3.1.1; HEL-S-49; TPID; TPI; TIM; Triosephosphate isomerase; Triose-phosphate isomerase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 5 µg/ml
WB0.01 - 0.03 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21991 Mouse TPI1

GeneID: 7167 Human TPI1

Swiss-port # P17751 Mouse Triosephosphate isomerase

Swiss-port # P60174 Human Triosephosphate isomerase

Background This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 31 kDa
PTM The initiator methionine for isoform 2 is removed.