ARG66849

anti-TrkC phospho (Tyr516) antibody

anti-TrkC phospho (Tyr516) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes TrkC phospho (Tyr516)
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity This antibody detects endogenous levels of TrkC protein only when phosphorylated at Tyr516.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TrkC
Antigen Species Human
Immunogen Phosphospecific peptide around Tyr516 (aa. 482-531) of Human TrkC.
Conjugation Un-conjugated
Alternate Names TRKC; Neurotrophic tyrosine kinase receptor type 3; Trk-C; TrkC tyrosine kinase; NT-3 growth factor receptor; GP145-TrkC; EC 2.7.10.1; gp145(trkC)

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:300
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa + serum
Observed Size ~ 150 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4916 Human NTRK3

Swiss-port # Q16288 Human NT-3 growth factor receptor

Gene Symbol NTRK3
Gene Full Name neurotrophic tyrosine kinase, receptor, type 3
Background This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Function Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 94 kDa
PTM Ligand-mediated auto-phosphorylation. [UniProt]