ARG67154
anti-Twist 1 antibody
anti-Twist 1 antibody for Western blot,ICC/IF and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Twist 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Twist 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide correspond to N-terminal region of human TWIST1. |
| Conjugation | Un-conjugated |
| Alternate Names | TWIST1; Twist Family BHLH Transcription Factor 1; H-Twist; BHLHa38; BPES2; TWIST; CRS1; SCS; Twist Basic Helix-Loop-Helix Transcription Factor 1; Class A Basic Helix-Loop-Helix Protein 38; Twist-Related Protein 1; BPES3; ACS3; CRS; Blepharophimosis, Epicanthus Inversus And Ptosis 3; Twist Homolog 1 (Drosophila); TWIST Homolog Of Drosophila; B-HLH DNA Binding Protein; Saethre-Chotzen Syndrome; Acrocephalosyndactyly 3; Craniosynostosis; Twist Homolog 1; BHLHA38; SWCOS; CSO |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TWIST1 |
| Gene Full Name | Twist Family BHLH Transcription Factor 1 |
| Background | This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Jul 2020] |
| Function | Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-BMAL1 heterodimer. [Uniprot] |
| Cellular Localization | Nucleus. [Uniprot] |
| Calculated MW | 21 kDa |
