ARG59179
anti-UBA1 / UBE1 antibody
anti-UBA1 / UBE1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes UBA1 / UBE1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Bov, Rb |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | UBA1 / UBE1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 1-30 of Human UBA1. |
| Conjugation | Un-conjugated |
| Alternate Names | UBE1; UBE1X; CFAP124; POC20; UBA1A; GXP1; A1S9; A1S9T; Ubiquitin-activating enzyme E1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; SMAX2; AMCX1; A1ST |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P22314 Human Ubiquitin-like modifier-activating enzyme 1 |
|---|---|
| Gene Symbol | UBA1 |
| Gene Full Name | ubiquitin-like modifier activating enzyme 1 |
| Background | The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites. [UniProt] |
| Cellular Localization | Cytoplasm. Mitochondrion. Nucleus. Isoform 1: Nucleus. Isoform 2: Cytoplasm. [UniProt] |
| Calculated MW | 118 kDa |
| PTM | ISGylated. [UniProt] |
