ARG59226
anti-UBA3 antibody
anti-UBA3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry,ICC/IF and Human,Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes UBA3 |
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Tested Reactivity | Hu, Ms, Rat |
Predict Reactivity | Bov, Dog, Hrs, Mk, Rb |
Tested Application | FACS, ICC/IF, IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | UBA3 |
Antigen Species | Human |
Immunogen | Synthetic peptide corresponding to aa. 409-448 of Human UBA3. (KNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVAD) |
Conjugation | Un-conjugated |
Alternate Names | EC 6.3.2.-; Ubiquitin-activating enzyme E1C; UBE1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3; NAE2; hUBA3; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C |
Application Instructions
Application Suggestion |
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Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
Preservative | 0.05% Sodium azide |
Stabilizer | 5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | UBA3 |
Gene Full Name | ubiquitin-like modifier activating enzyme 3 |
Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Function | Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. [UniProt] |
Calculated MW | 52 kDa |