ARG56851
anti-UBE2D1 antibody
anti-UBE2D1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes UBE2D1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Bov, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | UBE2D1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 111-140 (C-terminus) of Human UBE2D1. |
| Conjugation | Un-conjugated |
| Alternate Names | E2(17)KB1; EC 6.3.2.19; UBC4/5 homolog; Ubiquitin-conjugating enzyme E2; UbcH5; SFT; UBCH5; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2 D1; UBC4/5; Stimulator of Fe transport; Ubiquitin-protein ligase D1; Ubiquitin-conjugating enzyme E2-17 kDa 1; UBCH5A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A431 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by ammonium sulfate precipitation through ion exchange chromatography. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P51668 Human Ubiquitin-conjugating enzyme E2 D1 Swiss-port # P61080 Mouse Ubiquitin-conjugating enzyme E2 D1 |
|---|---|
| Gene Symbol | UBE2D1 |
| Gene Full Name | ubiquitin-conjugating enzyme E2D 1 |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. [UniProt] |
| Cellular Localization | Cytoplasm. |
| Calculated MW | 17 kDa |
