ARG63632

anti-UBE2I / UBC9 antibody

anti-UBE2I / UBC9 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody

Overview

Product Description Goat Polyclonal antibody recognizes UBE2I / UBC9
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name UBE2I / UBC9
Antigen Species Human
Immunogen SGIALSRLAQERK-C
Conjugation Un-conjugated
Alternate Names UBC9; Ubiquitin-protein ligase I; EC 6.3.2.-; SUMO-conjugating enzyme UBC9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; SUMO-protein ligase; p18; C358B7.1; P18; Ubiquitin carrier protein 9

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 µg/ml
WB0.1 - 0.3 µg/ml
Application Note IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25573 Rat UBE2I

GeneID: 7329 Human UBE2I

Swiss-port # P63279 Human SUMO-conjugating enzyme UBC9

Swiss-port # P63281 Rat SUMO-conjugating enzyme UBC9

Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 18 kDa
PTM Phosphorylation at Ser-71 significantly enhances SUMOylation activity.