ARG43060

anti-UBR2 antibody

anti-UBR2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes UBR2
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name UBR2
Antigen Species Human
Immunogen Recombinant protein corresponding to Q10-A664 of Human UBR2.
Conjugation Un-conjugated
Alternate Names C6orf133; bA49A4.1; EC 6.3.2.-; N-recognin-2; Ubiquitin-protein ligase E3-alpha-II; E3 ubiquitin-protein ligase UBR2; dJ392M17.3; Ubiquitin-protein ligase E3-alpha-2; dJ242G1.1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 224826 Mouse UBR2

GeneID: 23304 Human UBR2

Swiss-port # Q6WKZ8 Mouse E3 ubiquitin-protein ligase UBR2

Swiss-port # Q8IWV8 Human E3 ubiquitin-protein ligase UBR2

Gene Symbol UBR2
Gene Full Name ubiquitin protein ligase E3 component n-recognin 2
Background This gene encodes an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Function E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. Required for spermatogenesis, promotes, with Tex19.1, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis (By similarity). Polyubiquitinates LINE-1 retrotransposon encoded, LIRE1, which induces degradation, inhibiting LINE-1 retrotransposon mobilization (By similarity). [UniProt]
Cellular Localization Nucleus. Note=Associated with chromatin during meiosis. [UniProt]
Calculated MW 201 kDa