ARG54112

anti-UCHL1 / PGP9.5 antibody

anti-UCHL1 / PGP9.5 antibody for Western blot,ICC/IF and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody recognizes UCHL1
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Target Name UCHL1 / PGP9.5
Antigen Species Human
Immunogen Purified recombinant human UCHL1 / PGP9.5 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:300
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 25 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.2% Sodium azide, 50% Glycerol and 0.1%BSA
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol, 0.1%BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7345 Human UCHL1

Swiss-port # P09936 Human Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene Symbol UCHL1
Gene Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Background Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated_x000D_ proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of_x000D_ ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have_x000D_ ATP-independent ubiquitin ligase activity
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt]
Cellular Localization Cytoplasm
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
Calculated MW 25 kDa
PTM O-glycosylated.