ARG55836
anti-UCHL1 / PGP9.5 antibody
anti-UCHL1 / PGP9.5 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,ICC/IF and Human,Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes UCHL1 / PGP9.5 |
---|---|
Tested Reactivity | Hu, Ms, Rat |
Predict Reactivity | Bov, Mk, Pig |
Tested Application | FACS, ICC/IF, IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | UCHL1 / PGP9.5 |
Antigen Species | Human |
Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 187-216 (C-terminus) of Human UCHL1. |
Conjugation | Un-conjugated |
Alternate Names | PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12 |
Application Instructions
Application Suggestion |
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
Positive Control | SH-SY5Y |
Properties
Form | Liquid |
---|---|
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Buffer | PBS and 0.09% (W/V) Sodium azide |
Preservative | 0.09% (W/V) Sodium azide |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
---|---|
Gene Symbol | UCHL1 |
Gene Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
Background | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009] |
Function | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt] |
Cellular Localization | Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain |
Calculated MW | 25 kDa |
PTM | O-glycosylated. |