ARG42913

anti-UCHL5 / UCH37 antibody

anti-UCHL5 / UCH37 antibody for Western blot and Human

Overview

Product Description Mouse Monoclonal antibody recognizes UCHL5 / UCH37
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 854CT5.5.6
Isotype IgG1
Target Name UCHL5 / UCH37
Antigen Species Human
Immunogen Purified His-tagged Human UCHL5 / UCH37 protein.
Conjugation Un-conjugated
Alternate Names UCH-L5; CGI-70; UCH37; INO80R; Ubiquitin thioesterase L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin carboxyl-terminal hydrolase isozyme L5; EC 3.4.19.12

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:2000 - 1:4000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 35 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51377 Human UCHL5

Swiss-port # Q9Y5K5 Human Ubiquitin carboxyl-terminal hydrolase isozyme L5

Gene Symbol UCHL5
Gene Full Name ubiquitin carboxyl-terminal hydrolase L5
Function Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus. [UniProt]
Calculated MW 38 kDa