ARG54990
anti-ULK3 antibody
anti-ULK3 antibody for Western blot and Human,Mouse,Rat
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes ULK3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1404CT148.89.25 |
| Isotype | IgG1, kappa |
| Target Name | ULK3 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 435-468 of Human ULK3. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.7.11.1; Unc-51-like kinase 3; Serine/threonine-protein kinase ULK3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ULK3 |
| Gene Full Name | unc-51 like kinase 3 |
| Function | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. [UniProt] |
| Cellular Localization | Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 53 kDa |
| PTM | Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU. |
