ARG59528

anti-UNG antibody

anti-UNG antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes UNG
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name UNG
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-313 of Human UNG (NP_550433.1).
Conjugation Un-conjugated
Alternate Names Uracil-DNA glycosylase; HIGM4; HIGM5; UNG1; UNG2; UNG15; UDG; DGU; EC 3.2.2.27

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size 35 kDa, 40 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22256 Mouse UNG

GeneID: 7374 Human UNG

Swiss-port # P13051 Human Uracil-DNA glycosylase

Swiss-port # P97931 Mouse Uracil-DNA glycosylase

Gene Symbol UNG
Gene Full Name uracil DNA glycosylase
Background This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]
Function Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. [UniProt]
Cellular Localization Isoform 1: Mitochondrion. Isoform 2: Nucleus. [UniProt]
Calculated MW Isoform 1: 34 kDa
Isoform 2: 35 kDa
PTM Isoform 1 is processed by cleavage of a transit peptide. [UniProt]