ARG59528
anti-UNG antibody
anti-UNG antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse
Overview
Product Description | Rabbit Polyclonal antibody recognizes UNG |
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Tested Reactivity | Hu, Ms |
Tested Application | IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | UNG |
Antigen Species | Human |
Immunogen | Recombinant fusion protein corresponding to aa. 1-313 of Human UNG (NP_550433.1). |
Conjugation | Un-conjugated |
Alternate Names | Uracil-DNA glycosylase; HIGM4; HIGM5; UNG1; UNG2; UNG15; UDG; DGU; EC 3.2.2.27 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | HeLa | ||||||
Observed Size | 35 kDa, 40 kDa |
Properties
Form | Liquid |
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Purification | Affinity purified. |
Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | UNG |
Gene Full Name | uracil DNA glycosylase |
Background | This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010] |
Function | Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. [UniProt] |
Cellular Localization | Isoform 1: Mitochondrion. Isoform 2: Nucleus. [UniProt] |
Calculated MW | Isoform 1: 34 kDa Isoform 2: 35 kDa |
PTM | Isoform 1 is processed by cleavage of a transit peptide. [UniProt] |