ARG59722
anti-USP10 antibody
anti-USP10 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Immunoprecipitation and Human
Overview
Product Description | Rabbit Polyclonal antibody recognizes USP10 |
---|---|
Tested Reactivity | Hu |
Tested Application | ICC/IF, IHC-P, IP, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | USP10 |
Antigen Species | Human |
Immunogen | Synthetic peptide derived from Human USP10. |
Conjugation | Un-conjugated |
Alternate Names | Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; UBPO; Ubiquitin-specific-processing protease 10; Ubiquitin carboxyl-terminal hydrolase 10; EC 3.4.19.12 |
Application Instructions
Application Suggestion |
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
Observed Size | ~ 125 kDa |
Properties
Form | Liquid |
---|---|
Purification | Affinity purified. |
Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q14694 Human Ubiquitin carboxyl-terminal hydrolase 10 |
---|---|
Gene Symbol | USP10 |
Gene Full Name | ubiquitin specific peptidase 10 |
Background | Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013] |
Function | Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. [UniProt] |
Cellular Localization | Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions (PubMed:20096447). After DNA damage, translocates to the nucleus following phosphorylation by ATM (PubMed:20096447). [UniProt] |
Calculated MW | 87 kDa |
PTM | Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus. Ubiquitinated. Deubiquitinated by USP13. [UniProt] |