ARG54999
anti-USP14 antibody
anti-USP14 antibody for Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes USP14 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 889CT6.2.1 |
| Isotype | IgG1 |
| Target Name | USP14 |
| Immunogen | Purified His-tagged USP14 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Deubiquitinating enzyme 14; TGT; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14; Ubiquitin carboxyl-terminal hydrolase 14; EC 3.4.19.12 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Mouse brain |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P54578 Human Ubiquitin carboxyl-terminal hydrolase 14 Swiss-port # Q9JMA1 Mouse Ubiquitin carboxyl-terminal hydrolase 14 |
|---|---|
| Gene Symbol | USP14 |
| Gene Full Name | ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) |
| Background | This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 56 kDa |
