ARG63336

anti-USP16 / UBP-M antibody

anti-USP16 / UBP-M antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes USP16 / UBP-M
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application IHC-P
Specificity This antibody is expected to recognise isoform a (NP_006438.1) and isoform b (NP_001001992.1). Reported variants represent identical protein (NP_006438.1; NP_001027582.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name USP16 / UBP-M
Antigen Species Human
Immunogen GKKRTKGKTVPIDD-C
Conjugation Un-conjugated
Alternate Names UBP-M; Deubiquitinating enzyme 16; Ubiquitin-processing protease UBP-M; Ubiquitin thioesterase 16; Ubiquitin-specific-processing protease 16; UBPM; EC 3.4.19.12; Ubiquitin carboxyl-terminal hydrolase 16

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P4 - 6 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10600 Human USP16

Swiss-port # Q9Y5T5 Human Ubiquitin carboxyl-terminal hydrolase 16

Background This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 94 kDa
PTM Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.