ARG55001

anti-USP25 antibody

anti-USP25 antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody recognizes USP25
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 1277CT376.106.171
Isotype IgG1, kappa
Target Name USP25
Antigen Species Human
Immunogen Recombinant protein from Human USP25.
Conjugation Un-conjugated
Alternate Names USP on chromosome 21; Ubiquitin-specific-processing protease 25; Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; Ubiquitin thioesterase 25; USP21; EC 3.4.19.12

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Daudi

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29761 Human USP25

Swiss-port # Q9UHP3 Human Ubiquitin carboxyl-terminal hydrolase 25

Gene Symbol USP25
Gene Full Name ubiquitin specific peptidase 25
Background Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]
Function Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function. [UniProt]
Cellular Localization Cytoplasm
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 122 kDa
PTM Acetylated.
Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.