ARG54630
anti-Ubiquitin antibody
anti-Ubiquitin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoelectrophoresis,Immunoprecipitation,Western blot and Bovine,Chicken,D. melanogaster ,Dog,E. coli,Fish,Guinea pig,Hamster,Human,Monkey,Mouse,Pig,Rabbit,Rat,S. cerevisiae,Sheep,Xenopus laevis,Yeast
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
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Publication2
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Ubiquitin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Chk, Dm, Dog, E. coli, Fsh, Gpig, Hm, Mk, Pig, Rb, S. cerevisiae, Sheep, Xenopus laevis, Yeast |
| Tested Application | ICC/IF, IE, IHC-Fr, IHC-P, IP, WB |
| Specificity | This antibody will identify free ubiquitin aswell as ubiquinated proteins on western blot.It recognizes human, monkey, mouse, rat,hamster, rabbit guinea pig, bovine, porcine,canine, sheep, chicken, Xenopus, yeast,Drosophila, and fish ubiquitin. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Ubiquitin |
| Antigen Species | Bovine |
| Immunogen | Native bovine ubiquitin conjugated to KLH. |
| Conjugation | Un-conjugated |
| Alternate Names | Polyubiquitin-B |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-Fr: For PFA perfusion fixed frozen section. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Heat-shocked HeLa and Mouse brain |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A-purified antibody |
| Buffer | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | UBB |
| Gene Full Name | ubiquitin B |
| Background | Ubiquitin is a highly-conserved, 76-aminoacid polypeptide expressed in all eukaryoticcells. Ubiquitination of proteins facilitatestargeting of cytosolic and nuclear proteinsfor degradation by proteasomes. Thisessential cellular process involves a multienzymecascade of ubiquitin-activatingenzymes (E1s), ubiquitin-conjugatingenzymes (E2s or Ubcs), and ubiquitinproteinligases (E3s). Ubiquitination alsoparticipates in internalization anddegradation of plasma membrane proteinssuch as T cell receptor subunits while stillassociated with membranes of theendoplasmic reticulum. |
| Function | Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. [UniProt] |
| Highlight | Related products: Ubiquitin antibodies; Ubiquitin ELISA Kits; Ubiquitin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 26 kDa |
| PTM | Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291). |
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Review for anti-Ubiquitin antibody
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis
WB / Mouse
CD2-associated Protein Contributes to Hepatitis C Virus Propagation and Steatosis by Disrupting Insulin Signaling.
WB / Human
