ARG63849
anti-Urokinase / uPA antibody
anti-Urokinase / uPA antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
1
Overview
| Product Description | Goat Polyclonal antibody recognizes Urokinase / uPA |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Urokinase / uPA |
| Antigen Species | Human |
| Immunogen | C-HFLPWIRSHTKEEN |
| Conjugation | Un-conjugated |
| Alternate Names | ATF; uPA; U-plasminogen activator; BDPLT5; EC 3.4.21.73; QPD; URK; Urokinase-type plasminogen activator; u-PA; UPA |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. Approx 48 kDa band observed in lysates of human kidney fibroblast cell line HEK293 (calculated MW of 48.5 kDa according to NP_002649.1). A minor band was also consistently observed at appox 35 kDa. This band was successfully blocked by incubation with the immunising peptide, and is consistent with the B chain of Cathepsin B-mediated cleavage product of PLAU (Kobayashi et al, J Biol Chem. 1991 Mar 15;266(8):5147-52; PMID: 1900515). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P00749 Human Urokinase-type plasminogen activator |
|---|---|
| Gene Symbol | PLAU |
| Gene Full Name | plasminogen activator, urokinase |
| Background | This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] |
| Function | Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 49 kDa |
| PTM | Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. |
Customer's Feedback
Good
Review for anti-Urokinase / uPA antibody
Application：WB
Sample：HeLa, 293T and HCT116
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
