ARG62340

anti-V5 tag antibody [V5.E10]

anti-V5 tag antibody [V5.E10] for Dot blot,ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Gene Regulation antibody
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Overview

Product Description

Mouse Monoclonal antibody [V5.E10] recognizes V5 tag

Tested Reactivity Other
Tested Application Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity Recognizes V5-tagged fusion proteins
Host Mouse
Clonality Monoclonal
Clone V5.E10
Isotype IgG1
Target Name V5 tag
Antigen Species Others
Immunogen GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ELISAAssay-dependent
ICC/IF1:500-1:2000
IHC-Fr1:500-1:2000
IHC-P1:500-1:2000
IPAssay-dependent
WB1:1000-1:3000
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A Purified
Purification Note Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor.
Buffer 10mM PBS (pH 7.2) and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Both sequence is rarely be found in other proteins and can be used for protein analysis including binding assay, functional analysis and tracing the protein location in cells when the specific antibodies for the protein-of-interest are unavailable.
Highlight Related products:
V5 tag antibodies; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Gene Regulation antibody

Customer's Feedback

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Review for anti-V5 tag antibody [V5.E10]

Application:WB

Sample:293T and 293T + myc-V5-mCherry

Sample Loading Amount:10 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC