ARG66850
anti-VAV2 phospho (Tyr142) antibody
anti-VAV2 phospho (Tyr142) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes VAV2 phospho (Tyr142) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody detects endogenous levels of VAV2 protein only when phosphorylated at Tyr142. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VAV2 |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Tyr142 (aa. 108-157) of Human VAV2. |
| Conjugation | Un-conjugated |
| Alternate Names | Guanine nucleotide exchange factor VAV2; VAV-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | K562 + TNF HeLa + TNF |
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| Observed Size | ~ 100 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P52735 Human Guanine nucleotide exchange factor VAV2 Swiss-port # Q60992 Mouse Guanine nucleotide exchange factor VAV2 |
|---|---|
| Gene Symbol | VAV2 |
| Gene Full Name | vav 2 guanine nucleotide exchange factor |
| Background | VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| Function | Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). [UniProt] |
| Calculated MW | 101 kDa |
| PTM | Phosphorylated on tyrosine residues in response to FGR activation. [UniProt] |
