ARG20178
anti-VDAC1 / Porin antibody
anti-VDAC1 / Porin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Rabbit
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes VDAC1 / Porin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Rb |
| Tested Application | IHC-P, WB |
| Specificity | The antibody recognizes ~31 kDa VDAC1 / Porin from samples of human, mouse, rat, bovine, pig, and rabbit origins. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VDAC1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide surrounding amino acid 189 of human VDAC1 |
| Conjugation | Un-conjugated |
| Alternate Names | VDAC1; Voltage Dependent Anion Channel 1; PORIN; Voltage-Dependent Anion-Selective Channel Protein 1; Outer Mitochondrial Membrane Protein Porin 1; Plasmalemmal Porin; Porin 31HL; Porin 31HM; MGC111064; VDAC-1; Sperm Binding Protein 1a; HVDAC1; VDAC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Western Blot: 3T3 cell lysate. IHC: Liver tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified Antibody |
| Buffer | PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 30% Glycerol, 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VDAC1 |
| Gene Full Name | voltage-dependent anion channel 1 |
| Background | This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010] |
| Function | Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. [UniProt] |
| Cellular Localization | Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane. [UniProt] |
| Highlight | Related products: VDAC1 antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 31 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
Customer's Feedback
Good
Review for anti-VDAC1 / Porin antibody
Application：WB
Sample：HT29, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1 µg/ml
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-VDAC1 / Porin antibody
Application：WB
Sample：Rat liver
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-VDAC1 / Porin antibody
Application：WB
Sample：Mouse liver
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
