ARG41913

anti-VEGF antibody

anti-VEGF antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes VEGF
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application IHC-P, WB
Specificity Detects the 121, 165 and 189 VEGF isoforms.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name VEGF
Conjugation Un-conjugated
Alternate Names MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:300
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 16 - 45 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol.
Preservative 0.025% ProClin 300
Stabilizer 20% Glycerol
Concentration 1.56 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22339 Mouse VEGFA

GeneID: 7422 Human VEGFA

Swiss-port # P15692 Human Vascular endothelial growth factor A

Swiss-port # Q00731 Mouse Vascular endothelial growth factor A

Gene Symbol VEGFA
Gene Full Name vascular endothelial growth factor A
Background This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]
Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [UniProt]
Cellular Localization Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. [UniProt]
Highlight Related products:
VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Anti-Rabbit IgG secondary antibodies;
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Calculated MW 16 - 45 kDa