ARG42065

anti-VEGF antibody (Biotin)

anti-VEGF antibody (Biotin) for ELISA,Western blot and Human

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes VEGF
Tested Reactivity Hu
Tested Application ELISA, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name VEGF
Antigen Species Human
Immunogen Recombinant Human VEGF.
Conjugation Biotin
Alternate Names MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.25 - 1 µg/ml
WB0.1 - 0.2 µg/ml
Application Note Sandwich ELISA (Capture antibody - Detection antibody):
ARG42064 (0.5 - 2 µg/ml) - ARG42065 (0.25 - 1 µg/ml)

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7422 Human VEGFA

Swiss-port # P15692 Human Vascular endothelial growth factor A

Gene Symbol VEGFA
Gene Full Name vascular endothelial growth factor A
Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). [UniProt]
Cellular Localization Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. [UniProt]
Highlight Related products:
VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Anti-Goat IgG secondary antibodies;
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Calculated MW 22 kDa