ARG63885

anti-VPS25 antibody

anti-VPS25 antibody for Western blot and Human,Rat

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes VPS25
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Dog
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name VPS25
Antigen Species Human
Immunogen C-QPNVDTRQKQ
Conjugation Un-conjugated
Alternate Names EAP20; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25; Dermal papilla-derived protein 9; FAP20; Vacuolar protein-sorting-associated protein 25; hVps25; DERP9

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 681059 Rat VPS25

GeneID: 84313 Human VPS25

Swiss-port # P0C0A1 Rat Vacuolar protein-sorting-associated protein 25

Swiss-port # Q9BRG1 Human Vacuolar protein-sorting-associated protein 25

Background VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM, Mar 2008]
Research Area Signaling Transduction antibody
Calculated MW 21 kDa