ARG63417

anti-VPS28 antibody

anti-VPS28 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes VPS28
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog, Pig
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize isoform 1 (NP_057292.1) only.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name VPS28
Antigen Species Human
Immunogen C-ESAYNAFNRFLHA
Conjugation Un-conjugated
Alternate Names ESCRT-I complex subunit VPS28; H-Vps28; Vacuolar protein sorting-associated protein 28 homolog

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P10 µg/ml
WB1 - 3 µg/ml
Application Note IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51160 Human VPS28

Swiss-port # Q9UK41 Human Vacuolar protein sorting-associated protein 28 homolog

Background This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 25 kDa