ARG66199

anti-Vimentin antibody [SQab1721]

anti-Vimentin antibody [SQab1721] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
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publication_link Publication11

Overview

Product Description

Recombinant Rabbit Monoclonal antibody [SQab1721] recognizes Vimentin

Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1721
Target Name Vimentin
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Vimentin
Conjugation Un-conjugated
Alternate Names Vimentin; CTRCT30; HEL113

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100 - 1:500
ICC/IF1:10000 - 1:25000
IHC-FrAssay-dependent
IHC-P1:500 - 1:1000
IP1:50
WB1:1000 - 1:2000
Application Note IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
IHC-Fr: guaranteed based on publication PMID: 35011683
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22352 Mouse VIM

GeneID: 7431 Human VIM

Swiss-port # P08670 Human Vimentin

Swiss-port # P20152 Mouse Vimentin

Gene Symbol VIM
Gene Full Name vimentin
Background Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Highlight Related Antibody Duos and Panels:
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ARG30347 CAF Marker Antibody Panel
Related products:
Vimentin antibodies; Vimentin Duos / Panels;
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Anti-SerpinB9 therapy, a new strategy for cancer therapy
Research Area Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
Calculated MW 54 kDa
PTM Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Vimentin antibody [SQab1721]

Application:WB

Sample:HeLa and 293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Vimentin antibody [SQab1721]

Application:WB

Sample:COLO205, HCT116, HT29 and SW620

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Hypoxia-responsive circular RNA circAAGAB reduces breast cancer malignancy by activating p38 MAPK and sponging miR-378 h

WB, / Human

Kuan-Yi Lee et al.
Cancer Cell International,  (2023)

publication_link

 

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BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma

WB / Human

Mengna Li et al.
Cell Death Dis.,  (2023)

publication_link

 

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Yin Yang 1 suppresses tumor invasion and metastasis in nasopharyngeal carcinoma by negatively regulating eIF4E transcriptional activity and expression

WB / Human

Mengna Li et al.
Am J Cancer Res.,  (2023)

publication_link

 

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Perioperative Suppression of Schwann Cell Dedifferentiation Reduces the Risk of Adenomyosis Resulting from Endometrial-Myometrial Interface Disruption in Mice

IHC-P / Mouse

Xi Wang et al.
Biomedicines,  (2022)

publication_link

 

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Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells

WB, IHC-P / Human

Ya-Hui Chen et al.
Cancers (Basel).,  (2022)

publication_link

 

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Establishing F1A-CreER T2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes

IHC-Fr / Mouse

Yi-Chao Hsu et al.
Cells,  (2022)

publication_link

 

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Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains

IHC-P / Human

Keiji Wakamatsu et al.
Neuropathology,  (2022)

publication_link

 

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Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.

WB / Human

Jie Lian et al.
J Cancer.,  (2020)

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ACE2 Attenuates Epithelial-Mesenchymal Transition in MLE-12 Cells Induced by Silica.

WB / Mouse

Li Shumin et al.
Drug Des Devel Ther.,  (2020)

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A super-enhancer controls TGF- β signaling in pancreatic cancer through downregulation of TGFBR2.

WB / Human

Zhu Xiaolin et al.
Cell Signal.,  (2019)

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Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

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