ARG66199
anti-Vimentin antibody [SQab1721]
anti-Vimentin antibody [SQab1721] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
Publication11
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1721] recognizes Vimentin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1721 |
| Target Name | Vimentin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Vimentin |
| Conjugation | Un-conjugated |
| Alternate Names | Vimentin; CTRCT30; HEL113 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0 IHC-Fr: guaranteed based on publication PMID: 35011683 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VIM |
| Gene Full Name | vimentin |
| Background | Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017] |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30320 EMT Marker Antibody Panel ARG30322 Myofibroblast Differentiation Antibody Duo ARG30347 CAF Marker Antibody Panel Related products: Vimentin antibodies; Vimentin Duos / Panels; Related news: New antibody panels for Myofibroblasts and CAFs Cancer Pathology Markers (SQ clones) New EMT antibody panel is released New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| Research Area | Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody |
| Calculated MW | 54 kDa |
| PTM | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
Customer's Feedback
Excellent
Review for anti-Vimentin antibody [SQab1721]
Application：WB
Sample：HeLa and 293T
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Vimentin antibody [SQab1721]
Application：WB
Sample：COLO205, HCT116, HT29 and SW620
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Hypoxia-responsive circular RNA circAAGAB reduces breast cancer malignancy by activating p38 MAPK and sponging miR-378 h
WB, / Human
BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma
WB / Human
Yin Yang 1 suppresses tumor invasion and metastasis in nasopharyngeal carcinoma by negatively regulating eIF4E transcriptional activity and expression
WB / Human
Perioperative Suppression of Schwann Cell Dedifferentiation Reduces the Risk of Adenomyosis Resulting from Endometrial-Myometrial Interface Disruption in Mice
IHC-P / Mouse
Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells
WB, IHC-P / Human
Establishing F1A-CreER T2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes
IHC-Fr / Mouse
Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains
IHC-P / Human
Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.
WB / Human
ACE2 Attenuates Epithelial-Mesenchymal Transition in MLE-12 Cells Induced by Silica.
WB / Mouse
A super-enhancer controls TGF- β signaling in pancreatic cancer through downregulation of TGFBR2.
WB / Human
Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.
WB / Human
