ARG10662

anti-Vinculin antibody [HVIN-1 (VIN-54)]

anti-Vinculin antibody [HVIN-1 (VIN-54)] for IHC-Frozen sections,Western blot and Chicken,Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for cytoplasmic fraction; High MW loading control antibody

Overview

Product Description

Mouse Monoclonal antibody [HVIN-1 (VIN-54)] recognizes Vinculin

Tested Reactivity Hu, Ms, Rat, Chk
Tested Application IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone HVIN-1 (VIN-54)
Isotype IgG1
Target Name Vinculin
Antigen Species Human
Immunogen Human vinculin purified from uterus tissue.
Conjugation Un-conjugated
Alternate Names HEL114; Metavinculin; CMH15; Vinculin; CMD1W; MV; MVCL

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr2 - 4 µg/ml for formalin or acetone fixed tissues
WB1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Unpurified ascites
Buffer PBS, 0.01% Sodium azide and 1% BSA.
Preservative 0.01% Sodium azide
Stabilizer 1% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22330 Mouse VCL

GeneID: 305679 Rat VCL

GeneID: 396422 Chicken VCL

Gene Symbol VCL
Gene Full Name vinculin
Background Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. [UniProt]
Cellular Localization Cytoplasmic
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for cytoplasmic fraction; High MW loading control antibody
Calculated MW 124 kDa
PTM Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity).
Acetylated; mainly by myristic acid but also by a small amount of palmitic acid.