ARG22592
anti-WIPI2 antibody [2A2]
anti-WIPI2 antibody [2A2] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [2A2] recognizes WIPI2 This antibody recognizes WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2), also known as WIPI49-like protein 2. WIPI2 is a 454 amino acid ~54kDa autophagosomal marker containing three WD repeats. WIPI2 a mammalian orthologue of the yeast protein Atg18 and is similarly recruited to early autophagosomal structures and is required for their maturation into mature autophagosomes (Polson et al. 2010).Mouse anti Human WIPI2 antibody, clone 2A2 has been used for the immunofluorescent detection of WIPI2 in the human retinal epithelial cell line RPE1 (MacVicar and Lane 2014). |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2A2 |
| Isotype | IgG1 |
| Target Name | WIPI2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to the C-terminus of WIPI2b (CSALRLDEDSEHPPMILRTD) |
| Conjugation | Un-conjugated |
| Alternate Names | WD repeat domain phosphoinositide-interacting protein 2; WIPI-2; 2510001I10Rik; 1110018O08Rik |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q80W47 Mouse WD repeat domain phosphoinositide-interacting protein 2 Swiss-port # Q9Y4P8 Human WD repeat domain phosphoinositide-interacting protein 2 |
|---|---|
| Gene Symbol | Wipi2 |
| Gene Full Name | WD repeat domain, phosphoinositide interacting 2 |
| Function | Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Omegasomes are derived from ER and serve as intermediates for genesis of isolation membrane (also called preautophagosome) from ER cisternae membranes. [UniProt] |
| Calculated MW | 49 kDa |
