ARG42667
anti-WWP1 antibody
anti-WWP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes WWP1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | WWP1 |
| Antigen Species | Human |
| Immunogen | Fusion protein of Human WWP1. |
| Conjugation | Un-conjugated |
| Alternate Names | NEDD4-like E3 ubiquitin-protein ligase WWP1; WW domain-containing protein 1; EC 6.3.2.-; Atrophin-1-interacting protein 5; Tiul1; hSDRP1; AIP5; TGIF-interacting ubiquitin ligase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | WB: MDA-MB-231 and HeLa cell lysates IHC-P: Human esophagus cancer tissue |
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| Observed Size | ~ 110 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 0.4 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9H0M0 Human NEDD4-like E3 ubiquitin-protein ligase WWP1 |
|---|---|
| Gene Symbol | WWP1 |
| Gene Full Name | WW domain containing E3 ubiquitin protein ligase 1 |
| Background | WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined. [provided by RefSeq, Jul 2008] |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. [UniProt] |
| Calculated MW | 105 kDa |
| PTM | Auto-ubiquitinated and ubiquitinated by RNF11. [UniProt] |
