ARG62661

anti-XPA antibody [12F5]

anti-XPA antibody [12F5] for ICC/IF,Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [12F5] recognizes XPA
Tested Reactivity Hu
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 12F5
Isotype IgG2a, kappa
Target Name XPA
Antigen Species Human
Immunogen Recombinant human XPA protein.
Conjugation Un-conjugated
Alternate Names XP1; XPAC; Xeroderma pigmentosum group A-complementing protein; DNA repair protein complementing XP-A cells

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P1:400
WB1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Ls174T or MCF-7 cells or Human tonsil.

Properties

Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7507 Human XPA

Swiss-port # P23025 Human DNA repair protein complementing XP-A cells

Gene Symbol XPA
Gene Full Name xeroderma pigmentosum, complementation group A
Background This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
Function Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 31 kDa
PTM ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage.
Ubiquitinated by HERC2 leading to degradation by the proteasome.

Clone References

Nucleotide excision repair is not induced in human embryonic lung fibroblasts treated with environmental pollutants.

WB / 

Rossner P Jr et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Nucleotide excision repair is associated with the replisome and its efficiency depends on a direct interaction between XPA and PCNA.

ICC/IF / Human

Gilljam KM et al.
PLoS One.,  (2012)

publication_link

 

hr_line

Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repair.

WB, ICC/IF / Human

King BS et al.
J Biol Chem.,  (2012)

publication_link

 

hr_line

Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA.

WB / Human

Gueranger Q et al.
Nucleic Acids Res.,  (2011)

publication_link

 

hr_line

Recognition of cisplatin-DNA interstrand cross-links by replication protein A.

WB / Bovine

Patrick SM et al.
Biochemistry.,  (2008)

publication_link

 

hr_line

DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts.

IP / Human

Rytkönen AK et al.
FEBS J.,  (2006)

publication_link

 

hr_line

Expression of xeroderma pigmentosum A protein predicts improved outcome in metastatic ovarian carcinoma.

IHC / Human

Stevens EV et al.
Cancer.,  (2005)

publication_link

 

hr_line