ARG59617

anti-XPB antibody

anti-XPB antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes XPB
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name XPB
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 512-782 of Human XPB (NP_000113.1).
Conjugation Un-conjugated
Alternate Names TFIIH 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TTD2; RAD25; TFIIH p89; TFIIH basal transcription factor complex 89 kDa subunit; BTF2; Basic transcription factor 2 89 kDa subunit; XPB; BTF2 p89; DNA repair protein complementing XP-B cells; Xeroderma pigmentosum group B-complementing protein; EC 3.6.4.12; TFIIH; GTF2H; DNA excision repair protein ERCC-3

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat kidney, Mouse kidney and Jurkat
Observed Size 89 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13872 Mouse ERCC3

GeneID: 2071 Human ERCC3

GeneID: 291703 Rat ERCC3

Gene Symbol ERCC3
Gene Full Name excision repair cross-complementation group 3
Background This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Function ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 89 kDa