ARG62663

anti-XRCC1 antibody [33-2-5]

anti-XRCC1 antibody [33-2-5] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Hamster,Rat

Cancer antibody; Gene Regulation antibody; Metabolism antibody

Overview

Product Description Mouse Monoclonal antibody [33-2-5] recognizes XRCC1
Tested Reactivity Hu, Rat, Hm
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone 33-2-5
Isotype IgG2b
Target Name XRCC1
Antigen Species Human
Immunogen Recombinant full length protein (Human).
Conjugation Un-conjugated
Alternate Names X-ray repair cross-complementing protein 1; DNA repair protein XRCC1; RCC

Application Instructions

Application Note Flow Cyt: 1 µg for 10^6 cells.
ICC/IF: 1/200.
IHC-Fr: 1/50 - 1/100.
IHC-P: 1/50 - 1/100.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Testis

Properties

Form Liquid
Purification Protein A purified
Buffer PBS, 1% BSA and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 1% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7515 Human XRCC1

GeneID: 84495 Rat XRCC1

Swiss-port # P18887 Human DNA repair protein XRCC1

Swiss-port # Q9ESZ0 Rat DNA repair protein XRCC1

Gene Symbol XRCC1
Gene Full Name X-ray repair complementing defective repair in Chinese hamster cells 1
Background The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
Function Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. [UniProt]
Cellular Localization Nucleus.
Research Area Cancer antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 69 kDa
PTM Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated.

Clone References

DNA breaks and chromosomal aberrations arise when replication meets base excision repair.

Ensminger M et al.
J Cell Biol.,  (2014)

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PARP1-TDP1 coupling for the repair of topoisomerase I-induced DNA damage.

Das BB et al.
Nucleic Acids Res.,  (2014)

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PCAT-1, a long noncoding RNA, regulates BRCA2 and controls homologous recombination in cancer.

WB / 

Prensner JR et al.
Cancer Res.,  (2014)

publication_link

 

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Evidence that active demethylation mechanisms maintain the genome of carcinoma in situ cells hypomethylated in the adult testis.

Kristensen DG et al.
Br J Cancer.,  (2014)

publication_link

 

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Targeting XRCC1 deficiency in breast cancer for personalized therapy.

WB / Human

Sultana R et al.
Cancer Res.,  (2013)

publication_link

 

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Clinicopathological and functional significance of XRCC1 expression in ovarian cancer.

IHC / Human

Abdel-Fatah T et al.
Int J Cancer.,  (2013)

publication_link

 

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HMGN1 protein regulates poly(ADP-ribose) polymerase-1 (PARP-1) self-PARylation in mouse fibroblasts.

ICC/IF / Mouse

Masaoka A et al.
J Biol Chem.,  (2012)

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DNA mismatch repair proteins are required for efficient herpes simplex virus 1 replication.

WB / Rat

Mohni KN et al.
J Virol.,  (2011)

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Base excision repair processing of abasic site/single-strand break lesions within clustered damage sites associated with XRCC1 deficiency.

IP / Chinese Hamster

Mourgues S et al.
Nucleic Acids Res.,  (2007)

publication_link

 

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In situ analysis of repair processes for oxidative DNA damage in mammalian cells.

ICC/IF / Human

Lan L et al.
Proc Natl Acad Sci U S A.,  (2004)

publication_link

 

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Oxidative DNA damage and repair in experimental atherosclerosis are reversed by dietary lipid lowering.

WB / Rabbit

Martinet W et al.
Circ Res.,  (2001)

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