ARG62663
anti-XRCC1 antibody [33-2-5]
anti-XRCC1 antibody [33-2-5] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Hamster,Rat
Cancer antibody; Gene Regulation antibody; Metabolism antibody
Overview
Product Description | Mouse Monoclonal antibody [33-2-5] recognizes XRCC1 |
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Tested Reactivity | Hu, Rat, Hm |
Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
Host | Mouse |
Clonality | Monoclonal |
Clone | 33-2-5 |
Isotype | IgG2b |
Target Name | XRCC1 |
Antigen Species | Human |
Immunogen | Recombinant full length protein (Human). |
Conjugation | Un-conjugated |
Alternate Names | X-ray repair cross-complementing protein 1; DNA repair protein XRCC1; RCC |
Application Instructions
Application Note | Flow Cyt: 1 µg for 10^6 cells. ICC/IF: 1/200. IHC-Fr: 1/50 - 1/100. IHC-P: 1/50 - 1/100. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Positive Control | Testis |
Properties
Form | Liquid |
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Purification | Protein A purified |
Buffer | PBS, 1% BSA and 0.05% Sodium azide |
Preservative | 0.05% Sodium azide |
Stabilizer | 1% BSA |
Concentration | 0.2 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | XRCC1 |
Gene Full Name | X-ray repair complementing defective repair in Chinese hamster cells 1 |
Background | The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008] |
Function | Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. [UniProt] |
Cellular Localization | Nucleus. |
Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody |
Calculated MW | 69 kDa |
PTM | Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated. |
Clone References