ARG59150

anti-XRCC4 antibody

anti-XRCC4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes XRCC4
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name XRCC4
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-336 of Human XRCC4 (NP_071801.1).
Conjugation Un-conjugated
Alternate Names DNA repair protein XRCC4; SSMED; X-ray repair cross-complementing protein 4

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
IHC-P1:50 - 1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7518 Human XRCC4

Swiss-port # Q13426 Human DNA repair protein XRCC4

Gene Symbol XRCC4
Gene Full Name X-ray repair complementing defective repair in Chinese hamster cells 4
Background The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq, Sep 2008]
Function Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 38 kDa
PTM Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.

Monoubiquitinated.

Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination. [UniProt]