ARG59525

anti-ZAK antibody

anti-ZAK antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ZAK
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ZAK
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 100-312 of Human ZAK (NP_598407.1).
Conjugation Un-conjugated
Alternate Names AZK; MLK-related kinase; mlklak; EC 2.7.11.25; MLK7; MLK-like mitogen-activated protein triple kinase; MLTK; Human cervical cancer suppressor gene 4 protein; HCCS-4; MLT; MRK; Mitogen-activated protein kinase kinase kinase MLT; pk; Sterile alpha motif- and leucine zipper-containing kinase AZK; Leucine zipper- and sterile alpha motif-containing kinase; Mixed lineage kinase-related kinase

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart and Rat heart
Observed Size 105 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 65964 Mouse ZAK

Swiss-port # Q9ESL4 Mouse Mitogen-activated protein kinase kinase kinase MLT

Gene Symbol ZAK
Gene Full Name sterile alpha motif and leucine zipper containing kinase AZK
Background This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Function Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Translocates to the nucleus upon ultraviolet B irradiation. [UniProt]
Calculated MW 91 kDa