ARG42343

anti-acidic Cytokeratin antibody [AE1] (PE)

anti-acidic Cytokeratin antibody [AE1] (PE) for Flow cytometry and Human,Mouse,Rat,Cat,Chicken,Dog,Primates,Rabbit

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [AE1] recognizes acidic Cytokeratin
Tested Reactivity Hu, Ms, Rat, Cat, Chk, Dog, NHuPrm, Rb
Tested Application FACS
Specificity Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia.
Host Mouse
Clonality Monoclonal
Clone AE1
Isotype IgG1
Target Name acidic Cytokeratin
Antigen Species Human
Immunogen Human epidermal keratins.
Conjugation PE
Alternate Names KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16661 Mouse KRT10

GeneID: 3858 Human KRT10

GeneID: 450225 Rat KRT10

Gene Symbol KRT10
Gene Full Name keratin 10, type I
Background This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]
Function Plays a role in the establishment of the epidermal barrier on plantar skin.

(Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.

(Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur. [UniProt]
Cellular Localization Secreted, extracellular space. Note=Localized on the surface of desquamated nasal epithelial cells. [UniProt]
Calculated MW 59 kDa