ARG22217

anti-alpha B Crystallin antibody

anti-alpha B Crystallin antibody for ICC/IF,Immunohistochemistry,Western blot and Bovine,Chicken,Human,Mouse,Rat

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Overview

Product Description

Rabbit Polyclonal antibody recognizes alpha B Crystallin

Tested Reactivity Hu, Ms, Rat, Bov, Chk
Tested Application ICC/IF, IHC, WB
Specificity Detects ~22kDa. Does not cross-react with αA-crystallin.
Host Rabbit
Clonality Polyclonal
Target Name alpha B Crystallin
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide from Human alpha B crystallin
Conjugation Un-conjugated
Alternate Names HSPB5; Rosenthal fiber component; Renal carcinoma antigen NY-REN-27; MFM2; Alpha; CTRCT16; B; HspB5; Heat shock protein beta-5; HEL-S-101; CTPP2; CMD1II; CRYA2; Alpha-crystallin B chain

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:120
IHCAssay-dependent
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Rabbit antiserum.
Buffer Rabbit Antiserum
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12955 Mouse CRYAB

GeneID: 1410 Human CRYAB

GeneID: 25420 Rat CRYAB

Gene Symbol CRYAB
Gene Full Name crystallin, alpha B
Background Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. [UniProt]
Cellular Localization Cytoplasm, Nucleus
Calculated MW 20 kDa

Customer's Feedback

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Review for anti-alpha B Crystallin antibody

Application:WB

Sample:Rat heart

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC