ARG64364

anti-alpha B Crystallin antibody

anti-alpha B Crystallin antibody for Western blot and Mouse,Rat

Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes alpha B Crystallin
Tested Reactivity Ms, Rat
Predict Reactivity Hu
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name alpha B Crystallin
Antigen Species Human
Immunogen C-RLEKDRFSVNLD
Conjugation Un-conjugated
Alternate Names HSPB5; Rosenthal fiber component; Renal carcinoma antigen NY-REN-27; MFM2; Alpha; CTRCT16; B; HspB5; Heat shock protein beta-5; HEL-S-101; CTPP2; CMD1II; CRYA2; Alpha-crystallin B chain

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12955 Mouse CRYAB

GeneID: 25420 Rat CRYAB

Swiss-port # P23927 Mouse Alpha-crystallin B chain

Swiss-port # P23928 Rat Alpha-crystallin B chain

Background Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 20 kDa