ARG51802

anti-alpha Catenin phospho (Ser641) antibody

anti-alpha Catenin phospho (Ser641) antibody for Western blot and Human,Mouse

Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes alpha Catenin phospho (Ser641)
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name alpha Catenin
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 641 (D-D-S(p)-D-F) derived from Human Catenin alpha.
Conjugation Un-conjugated
Alternate Names Catenin alpha-1; Renal carcinoma antigen NY-REN-13; Cadherin-associated protein; CAP102; Alpha E-catenin

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12385 Mouse CTNNA1

GeneID: 1495 Human CTNNA1

Swiss-port # P26231 Mouse Catenin alpha-1

Swiss-port # P35221 Human Catenin alpha-1

Gene Symbol CTNNA1
Gene Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Background Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. May play a crucial role in cell differentiation.
Function Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. [UniProt]
Research Area Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 100 kDa
PTM Sumoylated.
Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion.