ARG59664
anti-alpha amylase antibody
anti-alpha amylase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes alpha amylase. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Hm |
| Tested Application | IHC-P, WB |
| Specificity | Sequence blast result indicats this antibody might react to alpha amylase 1, alpha amylase 2B and pancreatic alpha amylase (AMY2A). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | alpha amylase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 30-44 of Human alpha amylase. (HLFEWRWVDIALECE) |
| Conjugation | Un-conjugated |
| Alternate Names | 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY1; EC 3.2.1.1; Alpha-amylase 1; PA; AMY2; HXA; AMY2; AMY3 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | AMY1A; AMY2A; AMY2B |
|---|---|
| Gene Full Name | amylase, alpha 1A (salivary); amylase, alpha 2A (pancreatic); amylase, alpha 2B (pancreatic) |
| Background | Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 58 kDa |
Images (2) Click the Picture to Zoom In
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ARG59664 anti-alpha amylase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59664 anti-alpha amylase antibody at 1 µg/ml, overnight at 4°C.
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ARG59664 anti-alpha amylase antibody WB image
Western blot: 50 µg of samples under reducing conditions. PANC-1, Rat pancreas and Mouse pancreas lysates stained with ARG59664 anti-alpha amylase antibody at 0.5 µg/ml, overnight at 4°C.
