ARG63621

anti-alpha smooth muscle Actin antibody

anti-alpha smooth muscle Actin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
nuts_pic
1
publication_link Publication2

Overview

Product Description

Goat Polyclonal antibody recognizes alpha smooth muscle Actin

Tested Reactivity Hu, Ms
Predict Reactivity Dog, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name alpha smooth muscle Actin
Antigen Species Human
Immunogen EEEDSTALVC
Conjugation Un-conjugated
Alternate Names Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay - dependent
IHC-FrAssay - dependent
IHC-PAssay - dependent
WB0.1 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11475 Mouse ACTA2

GeneID: 59 Human ACTA2

Swiss-port # P62736 Human Actin, aortic smooth muscle

Swiss-port # P62737 Mouse Actin, aortic smooth muscle

Background The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Highlight Related Antibody Duos and Panels:
ARG30051 Smooth Muscle Marker Antibody Duo (Desmin, SMA)
ARG30328 Angiogenesis Antibody Panel
Related products:
alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Goat IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
Besides tumor suppression, what’s p53 busy for during embryogenesis?
New antibody panels and duos for Tumor immune microenvironment
Anti-SerpinB9 therapy, a new strategy for cancer therapy
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
Calculated MW 42 kDa
PTM Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Customer's Feedback

nuts_pic      Good

Review for anti-alpha smooth muscle Actin antibody

Application:WB

Sample:MEF cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:300

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Atherogenesis in Apoe−/− and Ldlr−/− Mice with a Genetically Resistant Background

IHC-Fr / Mouse

Hideyuki Torikai et al.
Cells,  (2023)

publication_link

 

hr_line

Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain.

IHC-Fr / Mouse

Jian Zhao et al.
Physiol Rep.,  (2021)

publication_link

 

hr_line