ARG56201

anti-basic Cytokeratin antibody [SPM591]

anti-basic Cytokeratin antibody [SPM591] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Mouse Monoclonal antibody [SPM591] recognizes basic Cytokeratin
Tested Reactivity Hu
Tested Application IHC-P
Host Mouse
Clonality Monoclonal
Clone SPM591
Isotype IgG1, kappa
Target Name basic Cytokeratin
Antigen Species Human
Immunogen Solubilized keratin extract from human stratum corneum.
Conjugation Un-conjugated
Alternate Names Keratin-76; KRT2P; CK-2P; Keratin, type II cytoskeletal 2 oral; Type-II keratin Kb9; HUMCYT2A; Cytokeratin-2P; K76; KRT2B; K2P

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51350 Human KRT76

Swiss-port # Q01546 Human Keratin, type II cytoskeletal 2 oral

Gene Symbol KRT76
Gene Full Name keratin 76, type II
Background Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009]
Function Probably contributes to terminal cornification. [UniProt]
Cellular Localization Cytoplasmic
Calculated MW 66 kDa