ARG62678
anti-beta 2 Microglobulin antibody [B2M-01]
anti-beta 2 Microglobulin antibody [B2M-01] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Radioimmunoassay,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [B2M-01] recognizes beta 2 Microglobulin |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms, Bov, Chk, Dog, Rb |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, IP, RIA, WB |
| Specificity | The clone B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B2M-01 |
| Isotype | IgG2a |
| Target Name | beta 2 Microglobulin |
| Antigen Species | Human |
| Immunogen | Purified human beta2-microglobulin |
| Conjugation | Un-conjugated |
| Alternate Names | Beta-2-microglobulin |
Application Instructions
| Application Suggestion |
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| Application Note | IP: The B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells. WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing condition. RIA: The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | FACS: Positive control: Peripheral blood lymphocytes. Negative control: Daudi and erythrocytes. WB: Positive control: U937 and Raji. Negative control: EL4 mouse lymphoblastic lymphoma cell line. IHC-P: Positive tissue: Kidney glomeruli. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by sequential precipitation methods with caprylic acid and ammonium sulphate. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | B2M |
| Gene Full Name | beta-2-microglobulin |
| Background | Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. |
| Function | Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody |
| Calculated MW | 14 kDa |
| PTM | Glycation of Ile-21 is observed in long-term hemodialysis patients. |
Clone References
Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.
FcRn binding properties of an abnormal truncated analbuminemic albumin variant.
ELISA / Human
Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B.
WB / Human
Oncostatin M enhances the antiviral effects of type I interferon and activates immunostimulatory functions in liver epithelial cells.
WB / Human
Beta2-microglobulin as a biomarker in peripheral arterial disease: proteomic profiling and clinical studies.
Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types.
