ARG62680
anti-beta 2 Microglobulin antibody [B2M-02]
anti-beta 2 Microglobulin antibody [B2M-02] for ELISA,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Pig
Cancer antibody; Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [B2M-02] recognizes beta 2 Microglobulin |
|---|---|
| Tested Reactivity | Hu, Pig |
| Tested Application | ELISA, FACS, IHC-P, WB |
| Specificity | The clone B2M-02 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes._x000D_ |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B2M-02 |
| Isotype | IgG1 |
| Target Name | beta 2 Microglobulin |
| Antigen Species | Human |
| Immunogen | Purified isolated human beta2-microglobulin. |
| Conjugation | Un-conjugated |
| Alternate Names | Beta-2-microglobulin |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | FACS: Positive control: PBL cell line. Negative control: Daudi and erythrocytes. WB: Positive control: U937. IHC-P: Positive tissue: Liver. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | B2M |
| Gene Full Name | beta-2-microglobulin |
| Background | Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. |
| Function | Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody |
| Calculated MW | 14 kDa |
| PTM | Glycation of Ile-21 is observed in long-term hemodialysis patients. |
Clone References
A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells.
The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin.
